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spots function  (Oxford Instruments)


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    Structured Review

    Oxford Instruments spots function
    Spots Function, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spots+function/Imaris/pm42151476-216-6-9
    Average 99 stars, based on 44196 article reviews
    spots function - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    other:

    Article Title: Tofacitinib repairs inflammation and mitochondrial dysregulation in GM-CSF-reprogrammed RA macrophages
    Article Snippet: The Spots function in Imaris was used to quantify the signal of interest (TOM20) using an estimated spot diameter of 0.75 μm.

    Article Title: Tofacitinib repairs inflammation and mitochondrial dysregulation in GM-CSF-reprogrammed RA macrophages.
    Article Snippet: The Spots function in Imaris was used to quantify the signal of interest (TOM20) using an estimated spot diameter of 0.75 μm.

    Microscopy:

    Article Title: Purpose-Adaptable Reinforced 3D Hyaluronic-Acid Based Platform to Study Pathomechanisms of the Central Nervous System.
    Article Snippet: 3D samples were incubated at room temperature for 30 min with Calcein-AM (1:500, green/living cells; Thermo Fisher Scientific, USA) and ethidium homodimer (1:1000, red/dead cells; Sigma–Aldrich, USA) diluted in PBS (pH7.4). .. Samples were imaged using a confocal microscope (Olympus IX81, Japan), and the live/dead ratio was analyzed Advanced Healthcare Materials, 2026 using the Spots function in Imaris Software 10.4 (Oxford Instrumentals, UK). ..

    Imaging:

    Article Title: B lymphocytes that enter the germinal center late preferentially differentiate into memory cells that recognize subdominant epitopes.
    Article Snippet: Microscopy was performed on an inverted laser scanning microscope (LSM980, Zeiss). .. For quantification, the Spots function in Imaris imaging software (Bitplane) was used to identify zsGreen + germinal center B cells (GCs) based on zsGreen fluorescence intensity and cell quality (a metric automatically quantified by the software). ..

    Software:

    Article Title: B lymphocytes that enter the germinal center late preferentially differentiate into memory cells that recognize subdominant epitopes.
    Article Snippet: Microscopy was performed on an inverted laser scanning microscope (LSM980, Zeiss). .. For quantification, the Spots function in Imaris imaging software (Bitplane) was used to identify zsGreen + germinal center B cells (GCs) based on zsGreen fluorescence intensity and cell quality (a metric automatically quantified by the software). ..

    Fluorescence:

    Article Title: B lymphocytes that enter the germinal center late preferentially differentiate into memory cells that recognize subdominant epitopes.
    Article Snippet: Microscopy was performed on an inverted laser scanning microscope (LSM980, Zeiss). .. For quantification, the Spots function in Imaris imaging software (Bitplane) was used to identify zsGreen + germinal center B cells (GCs) based on zsGreen fluorescence intensity and cell quality (a metric automatically quantified by the software). ..

    Functional Assay:

    Article Title: Dopaminergic modulation of low- and high spontaneous rate-type I auditory nerve fiber activity.
    Article Snippet: 1 Daniel O. J. Reijntjes*, Marco Manca*, Jingjing Sherry Wu, Philippe F.Y.. Vincent, Sharma Kushal, 2 Eunyoung Yi, Grace Tate, Kevin J Psoter and Elisabeth Glowatzki 3 4 The Johns Hopkins University School of Medicine, Department of Otolaryngology-Head and Neck 5 Surgery, The Center for Hearing and Balance, Baltimore, MD 21205, USA 6 Current address: Harvard Medical School, Blavatnik Institute, Department of Neurobiology, Boston, MA 7 02115, USA 8 Current address: Sound Pharmaceuticals, Inc., 4010 Stone Way N, #120.. Seattle, WA 98103 9 Mokpo National University, College of Pharmacy and Natural Medicine Research Institute 1



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    Oxford Instruments imaris spot tool function
    (A) Representative images from FiloQuant analysis used to quantify filopodia density in healthy and SMA patient astrocyte monocultures at baseline and after stimulating actin remodeling using the ATP depletion and recovery assay (NaN3 +R). CMFDA dye was used to visualize the cells (grey signal) and filopodia quantified by FiloQuant <t>tool</t> are highlighted in magenta. Scale bar: 30µm. (B) <t>Imaris</t> surface and mask tool applied on to individual healthy and SMA patient astrocyte cells at baseline and after ATP depletion and recovery assay. Scale bar: 15µm. (C) Quantification of filopodia density from FiloQuant analysis across baseline and treatment conditions for healthy and SMA patient astrocyte samples. One-way ANOVA with Bonferroni multiple comparison statistical testing; ****p<0.0001. (D) Optic density readout from CDC42-GTP G-LISA assay measuring the activated form of CDC42 across baseline and treatment conditions for healthy and SMA patient astrocyte samples. One-way ANOVA with Bonferroni multiple comparison statistical testing, p-values not statistically significant. N=5 (biological replicates), n= 4 (technical replicates).
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    Image Search Results


    (A) Representative images from FiloQuant analysis used to quantify filopodia density in healthy and SMA patient astrocyte monocultures at baseline and after stimulating actin remodeling using the ATP depletion and recovery assay (NaN3 +R). CMFDA dye was used to visualize the cells (grey signal) and filopodia quantified by FiloQuant tool are highlighted in magenta. Scale bar: 30µm. (B) Imaris surface and mask tool applied on to individual healthy and SMA patient astrocyte cells at baseline and after ATP depletion and recovery assay. Scale bar: 15µm. (C) Quantification of filopodia density from FiloQuant analysis across baseline and treatment conditions for healthy and SMA patient astrocyte samples. One-way ANOVA with Bonferroni multiple comparison statistical testing; ****p<0.0001. (D) Optic density readout from CDC42-GTP G-LISA assay measuring the activated form of CDC42 across baseline and treatment conditions for healthy and SMA patient astrocyte samples. One-way ANOVA with Bonferroni multiple comparison statistical testing, p-values not statistically significant. N=5 (biological replicates), n= 4 (technical replicates).

    Journal: bioRxiv

    Article Title: Astrocyte targeted SMN1 gene therapy and forskolin application improves astrocyte filopodia actin defects and motor neuron synaptic dysfunction in human SMA disease pathology

    doi: 10.64898/2026.03.26.714618

    Figure Lengend Snippet: (A) Representative images from FiloQuant analysis used to quantify filopodia density in healthy and SMA patient astrocyte monocultures at baseline and after stimulating actin remodeling using the ATP depletion and recovery assay (NaN3 +R). CMFDA dye was used to visualize the cells (grey signal) and filopodia quantified by FiloQuant tool are highlighted in magenta. Scale bar: 30µm. (B) Imaris surface and mask tool applied on to individual healthy and SMA patient astrocyte cells at baseline and after ATP depletion and recovery assay. Scale bar: 15µm. (C) Quantification of filopodia density from FiloQuant analysis across baseline and treatment conditions for healthy and SMA patient astrocyte samples. One-way ANOVA with Bonferroni multiple comparison statistical testing; ****p<0.0001. (D) Optic density readout from CDC42-GTP G-LISA assay measuring the activated form of CDC42 across baseline and treatment conditions for healthy and SMA patient astrocyte samples. One-way ANOVA with Bonferroni multiple comparison statistical testing, p-values not statistically significant. N=5 (biological replicates), n= 4 (technical replicates).

    Article Snippet: Using the Imaris spot tool function, we quantified the CD44 and pERM puncta within astrocyte filopodia ( ) which revealed a statistically significant decrease in pERM , CD44 , and co-localized CD44/pERM ( ) expression within SMA patient-derived astrocytes cultures compared to healthy astrocytes at baseline and after actin remodeling stimulation.

    Techniques: Comparison